Journal: Antioxidants

Article Title: Alkyl N -Benzylthiocarbamates, the First Copper(II) Ion-Chelating Tyrosinase Inhibitors with a Thiocarbamate Group and ROS-Scavenging Activity, Exhibit Different Inhibitory Activities Depending on the Origin of Tyrosinase

doi: 10.3390/antiox15010039

Figure Lengend Snippet: Cu 2+ chelation activity of NBTC analogs 1 – 10 determined using copper sulfate (209 μM) and pyrocatechol violet (PCV; 80 μM). The activities of their Cu 2+ chelation were contrasted with those of kojic acid (20 μM; KA). All test samples were tested at 100 μM. *** p < 0.001 vs. KA-treated group.

Article Snippet: CuSO 4 aqueous solution (6.27 mM, 10 μL) was added to each well of a 96-well plate that contained pyrocatechol violet (PCV) (4 mM, 6 μL; Tokyo Chemical Industry, Tokyo, Japan), NBTC analogs (10 μL, 3.06 mM; final concentration: 100 μM), and AcOH-AcONa buffer (pH 6.0, 50 mM; 280 μL).

Techniques: Activity Assay